1 ago. Vírus da artrite encefalite caprina: isolamento e caracterização de parte do gene gag. Article (PDF Available) in Arquivo Brasileiro de Medicina. Palavras-chave: Animal diseases, Artrite-encefalite Caprina, CAEV, Caprine arthritis encephalit virus, Caprino, DNA sequence, Disease transmission, Doença . Mycoplasma agalactiae em rebanhos leiteiros no estado do Ceará em associação com o vírus da artrite encefalite caprina.
|Published (Last):||27 April 2018|
|PDF File Size:||5.78 Mb|
|ePub File Size:||11.78 Mb|
|Price:||Free* [*Free Regsitration Required]|
This rights cover the whole data about this document as well as its contents. Learn what derived works are clicking here.
The transcervical washing proved to be potentially method to reduce the virus concentration in reproductive tract, since the amount of fluid infused in the uterus capdina reduce the concentration of free virus in reproductive tract. As control group, two CAEV-free goats were artificially inseminated with semen from same buck without viral inoculum; and other two CAEV goats inseminated with the viral inoculum.
However the embryos from this medium, in two parts: Any uses or copies of this document in whole or in part must include the author’s name. Show full item record.
The viral load used in the present study was able to infect the females. Study on the transmissibility of the caprine arthritis encephalitis virus through semen and placenta. Throughout the period investigated, no proviral DNA was identify in the recipients blood.
However it is not possible to assume the CAEV transmission to offspring indisputably, though is not discarded the possibility of infection through semen and placenta in offspring with delayed seroconversion. Reproduction for commercial use is forbidden.
Artrite Encefalite Caprina by Fábio Assis Menezes on Prezi
The nested-PCR technique failed in detecting the infection before seroconversion in females. Paula Maria Pires do Nascimento Orientador: Embryos recovered through transcervical flushing were subjected to inner cell mass puncture using a micromanipulador.
The study on the presence of the virus in the uterus these being selected as embryo donors.
Tissues studied also showed positive results for the presence of pro-virus and viruses, indicating that may be occurring viral replication in the reproductive tract. A second study was to investigate the presence of CAEV in the reproductive tract of naturally infected goats and a third the risk was conducted to evaluate the virus transmission by embryo transfer. The two parties of the embryos were individualized. Newborns acprina the four-month evaluation were also seronegative.
This study provided more information about the caprine lentivirus transmission concerning the reproductive aspect, using three different diagnostic methods. The occurrence of anti-CAEV antibodies in the studied properties was Throughout the gestational period five months and four postnatal months total blood was collected once a month of the recipients for CAEV diagnosing by Nested PCR method.
The study was conducted in steps, first a serological survey in five major producing properties of goat milk in the state of Minas Gerais, to identify the occurrence of anti-CAEV dncefalite. Romulo Cerqueira Leite Membro: The monitoring was also performed by the same method of diagnosis in animals born during the first four months of life.
Therefore to emphasizes the need to adopt new technologies on the properties as the separation of infected and healthy animals.
Regarding the reproductive behavior in the transmission, the objective of the present study consists of experimentally evaluate the transmissibility of caprine lentivirus in goats and their offspring through the placenta and semen. The present study did not show the transmission of CAEV by embryos from positive goats after trypsin washes.
Five properties located in different cities in the state of Minas Gerais were evaluated, totaling animals of different ages and races Toggenburg, Daprina and Saanen. Therefore, it can be inferred that embryos derived from naturally infected goats, even in the presence of pro-virus and virus free in uterine lumen are apparently not permissive to CAEV infection due to the presence of intact zone pellucida and transcervical flushing can reduce the risk of virus transmission.
According to the results, a high occurrence of seropositive animals was identified in caprrina state of Minas Gerais, which has one of the largest herds of dairy goats in Brazil. The exclusion of positive breeding in properties is also a measure of control, it has been demonstrated that these are an important route of artrrite.
The positivity found in offspring at nested-PCR may have a particular meaning in identify dncefalite but seronegative animals, as in late seroconversion situations.